RNA-SeQC is a program which provides key measures of data quality. These metrics include yield, alignment and 
duplication rates; GC bias, rRNA content, regions of alignment (exon, intron and intragenic), continuity of coverage, 3′/5′ bias and count of detectable transcripts, among others. The software provides multi-sample evaluation of library construction protocols, input materials and other experimental parameters. The modularity of the software enables pipeline integration and the routine monitoring of key measures of data quality such as the number of alignable reads, duplication rates and rRNA contamination. RNA-SeQC allows investigators to make informed decisions about sample inclusion in downstream analysis. In summary, RNA-SeQC provides quality control measures critical to experiment design, process optimization and downstream computational analysis.
Incoming search terms:
- RNA-SeQC alternative splicing
- RSEM output
- ngs rna seq
- RNA expression analyses
- rnaseq mapping
- rnaseq micro rna
- rnaseq Paired end Single Read
- transcriptomics
